Molecular modelling of S1 and S2 subunits of SARS coronavirus spike glycoprotein.
Identifieur interne : 005F27 ( Main/Exploration ); précédent : 005F26; suivant : 005F28Molecular modelling of S1 and S2 subunits of SARS coronavirus spike glycoprotein.
Auteurs : Ottavia Spiga [Italie] ; Andrea Bernini ; Arianna Ciutti ; Stefano Chiellini ; Nicola Menciassi ; Francesca Finetti ; Vincenza Causarono ; Francesca Anselmi ; Filippo Prischi ; Neri NiccolaiSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Glycoproteins, Viral Proteins.
- chemistry : SARS Virus.
- Amino Acid Sequence, Models, Molecular, Molecular Sequence Data, Sequence Homology, Amino Acid.
Abstract
The S1 and S2 subunits of the spike glycoprotein of the coronavirus which is responsible for the severe acute respiratory syndrome (SARS) have been modelled, even though the corresponding amino acid sequences were not suitable for tertiary structure predictions with conventional homology and/or threading procedures. An indirect search for a protein structure to be used as a template for 3D modelling has been performed on the basis of the genomic organisation similarity generally exhibited by coronaviruses. The crystal structure of Clostridium botulinum neurotoxin B appeared to be structurally adaptable to human and canine coronavirus spike protein sequences and it was successfully used to model the two subunits of SARS coronavirus spike glycoprotein. The overall shape and the surface hydrophobicity of the two subunits in the obtained models suggest the localisation of the most relevant regions for their activity.
DOI: 10.1016/j.bbrc.2003.08.122
PubMed: 14511651
Affiliations:
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Le document en format XML
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last="Ciutti">Arianna Ciutti</name></author><author><name sortKey="Chiellini, Stefano" sort="Chiellini, Stefano" uniqKey="Chiellini S" first="Stefano" last="Chiellini">Stefano Chiellini</name></author><author><name sortKey="Menciassi, Nicola" sort="Menciassi, Nicola" uniqKey="Menciassi N" first="Nicola" last="Menciassi">Nicola Menciassi</name></author><author><name sortKey="Finetti, Francesca" sort="Finetti, Francesca" uniqKey="Finetti F" first="Francesca" last="Finetti">Francesca Finetti</name></author><author><name sortKey="Causarono, Vincenza" sort="Causarono, Vincenza" uniqKey="Causarono V" first="Vincenza" last="Causarono">Vincenza Causarono</name></author><author><name sortKey="Anselmi, Francesca" sort="Anselmi, Francesca" uniqKey="Anselmi F" first="Francesca" last="Anselmi">Francesca Anselmi</name></author><author><name sortKey="Prischi, Filippo" sort="Prischi, Filippo" uniqKey="Prischi F" first="Filippo" last="Prischi">Filippo Prischi</name></author><author><name sortKey="Niccolai, Neri" sort="Niccolai, Neri" uniqKey="Niccolai N" first="Neri" last="Niccolai">Neri Niccolai</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Glycoproteins (chemistry)</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>SARS Virus (chemistry)</term><term>Sequence Homology, Amino Acid</term><term>Viral Proteins (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Glycoprotéines ()</term><term>Modèles moléculaires</term><term>Protéines virales ()</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Virus du SRAS 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corresponding amino acid sequences were not suitable for tertiary structure predictions with conventional homology and/or threading procedures. An indirect search for a protein structure to be used as a template for 3D modelling has been performed on the basis of the genomic organisation similarity generally exhibited by coronaviruses. The crystal structure of Clostridium botulinum neurotoxin B appeared to be structurally adaptable to human and canine coronavirus spike protein sequences and it was successfully used to model the two subunits of SARS coronavirus spike glycoprotein. The overall shape and the surface hydrophobicity of the two subunits in the obtained models suggest the localisation of the most relevant regions for their activity.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Anselmi, Francesca" sort="Anselmi, Francesca" uniqKey="Anselmi F" first="Francesca" last="Anselmi">Francesca Anselmi</name><name sortKey="Bernini, Andrea" sort="Bernini, Andrea" uniqKey="Bernini A" first="Andrea" last="Bernini">Andrea Bernini</name><name sortKey="Causarono, Vincenza" sort="Causarono, Vincenza" uniqKey="Causarono V" first="Vincenza" last="Causarono">Vincenza Causarono</name><name sortKey="Chiellini, Stefano" sort="Chiellini, Stefano" uniqKey="Chiellini S" first="Stefano" last="Chiellini">Stefano Chiellini</name><name sortKey="Ciutti, Arianna" sort="Ciutti, Arianna" uniqKey="Ciutti A" first="Arianna" last="Ciutti">Arianna Ciutti</name><name sortKey="Finetti, Francesca" sort="Finetti, Francesca" uniqKey="Finetti F" first="Francesca" last="Finetti">Francesca Finetti</name><name sortKey="Menciassi, Nicola" sort="Menciassi, Nicola" uniqKey="Menciassi N" first="Nicola" last="Menciassi">Nicola Menciassi</name><name sortKey="Niccolai, Neri" sort="Niccolai, Neri" uniqKey="Niccolai N" first="Neri" last="Niccolai">Neri Niccolai</name><name sortKey="Prischi, Filippo" sort="Prischi, Filippo" uniqKey="Prischi F" first="Filippo" last="Prischi">Filippo Prischi</name></noCountry><country name="Italie"><noRegion><name sortKey="Spiga, Ottavia" sort="Spiga, Ottavia" uniqKey="Spiga O" first="Ottavia" last="Spiga">Ottavia Spiga</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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